Figure 7

Draining gut and liver lymph node T-cell hematopoiesis. (a–f) 12 week-old male SPF & GF mice were euthanized; Mesenteric lymph node (MLN) and liver lymph node (LLN) cells were isolated and stained for flow cytometric analysis (n = 4/gp). (a) % CD3⁺CD4⁺CD8⁻ (helper) T-cells, (b) % CD3⁺CD4⁻CD8⁺ (cytotoxic) T-cells, and (c) % CD3⁺CD4⁻CD8⁻TCRγδ⁺ (gamma delta) T-cells are expressed relative to total lymph node cells. (d) % CD4⁺CD25⁺FOXP3⁺ (T_REG) cells, (e) % CD4⁺CD196⁺RORγt⁺ (T_H17) cells, and (f) % CD4⁺CD183⁺T-bet⁺ (T_H1) cells are expressed relative to CD4⁺ cells. Data are reported as mean ± SEM. **p < 0.01 vs. SPF; ***p < 0.001 vs. SPF.