Figure 4
From: Mass-spectrometry analysis of modifications at DNA termini induced by DNA polymerases
Gel-electrophoresis of the PE reactions in the presence of different exonuclease-deficient and proofreading DNA polymerases at 72 °C. The reaction was performed for 2 h at dNTP concentrations of 200 μM. The ability to modify the sequence ends decreases in the following order for non-proofreading polymerases: Taq > Vent (exo-) > DeepVent (exo-) > Bst 2.0. The Therminator polymerase yields poor primer extension. The proofreading polymerases Pfu and Phusion can modify the M strand via terminal dT/Y exchange.
