Figure 7
From: Mass-spectrometry analysis of modifications at DNA termini induced by DNA polymerases
MS analysis of the hairpin modification (H4) by the Phusion and Pfu polymerases at 64 °C for 2 hours in the presence of 200 μM nucleotide Y. Pfu polymerase can excise the terminal dT in the absence of PPi. Partially degraded hairpin Cy3-AGGGAGTTGGTCTGATT-TTCAGACC (**) was also detected in the spectrum. Nucleotide exchange was only observed in the presence of 0.4 mM PPi. Phusion polymerase can generate the H4-dT + Y product without PPi because of exonuclease and polymerase activities.
