Table 1 Products of polymerase-assisted terminal modification reactions with extendable and non-extendable models.
From: Mass-spectrometry analysis of modifications at DNA termini induced by DNA polymerases
Model | Polymerase | dNTPs | T, °C | Reaction product (MS peak) |
|---|---|---|---|---|
M:P | Taq | d(AGC), Y | 72/64 | M + dA (2), M-dT + Y (3), M + Y (4), M-dT + Y + dA (4), M-dT + Y + dG (4), M-dT + Y + Y (5), F (6) F + Y (7) |
M:P | Vent (exo-) | d(AGC), Y | 72/64 | F + Y (7), M-dT + Y (3), F + dA (9) |
M:P | D.Vent (exo-) | d(AGC), Y | 72 | F (6), M + dA (2), M-dT + Y (3) |
M:P | Bst 2.0 | d(AGC), Y | 72 | F (6) |
M:P | Therminator | d(AGC), Y | 72 | M + dA/dG (2), M + dA + dA (10), M + Y (4), M-dT + Y + dA (4), M + Y + dA (11), M-dT + Y + Y (5) |
M:P | Pfu | d(AGC), Y | 72 | M-dT + Y (3) |
M:P | Phusion | d(AGC), Y | 72 | M-dT + Y (3), P + CAGYCAGAGACGY (8) |
H1 | Taq | d(AGC) | 64 | H1 + dA |
H1 | Taq | Y | 64 | H1 + Y |
H1 | Taq | d(AGC), Y | 64 | H1 + Y |
H1/PPi | Taq | Y | 64 | H1-dT + Y |
H4/PPi | Taq | Y | 64 | H4-dT + Y |
H4 | Therminator | Y | 64 | H4 + Y |
H4/PPi | Therminator | Y | 64 | H4-dT + Y + Y |
H4 | Pfu | Y | 64 | H4-dT |
H4 | Phusion | Y | 64 | H4-dT + Y |
H4/PPi | Pfu | Y | 64 | H4-dT + Y |
H4/PPi | Phusion | Y | 64 | H4-dT + Y |
