Figure 3 | Scientific Reports

Figure 3

From: Endothelial Dysfunction in Severe Preeclampsia is Mediated by Soluble Factors, Rather than Extracellular Vesicles

Figure 3

Media analysis by ELISA for (A–C) PlGF, (D–F) sFlt-1 and (G–I) Endoglin from preterm, term and PE placenta. PlGF and sFlt-1 levels were mainly detected in total and EV-free media. Endoglin was detected in total media but its levels were markedly reduced in EV-depleted media. All three angiogenic proteins showed minimal levels in EV-enriched media, microvesicle-enriched media and EV lysates. Large micro-vesicles = obtained after the 10,000 g spin, include micro-vesicle and apoptotic bodies. EV lysates = obtained by RIPA lysis of purified EV pellets.

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