Figure 1 | Scientific Reports

Figure 1

From: Transcriptome profiling of mouse brains with qkI-deficient oligodendrocytes reveals major alternative splicing defects including self-splicing

Figure 1

Gene expression profiles comparing brains of QKI FL/FL;Olig2-Cre and QKI FL/FL;- mice. (a) Global effects of QKI FL/FL;Olig2-Cre on cells were evaluated by unsupervised clustering of samples based on expression profiles of 1,000 most variant genes. QKI FL/FL;Olig2-Cre and QKI FL/FL;- mice form robust, distinct clusters, both with hierarchical clustering (right) and Principal Component Analysis (left). (b) Volcano plot representing the results of differential expression analysis. Genes with adjusted p-value < 0.05, absolute fold change >2 and base mean >100 are colored in red. (c) Validation of RNA-seq data by qPCR with 19 transcripts. RNA-seq data strongly correlated with qPCR data (r2 = 0.8049, p < 0.0001, Pearson correlation coefficient).

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