Table 1 Allergenic protein families identified in feverfew pollen by proteomics.

From: Proteomic profiling of the weed feverfew, a neglected pollen allergen source

Identified allergenic protein families by (LC-MS/MS and 2-DE-LC-MS/MS)

Allergenic protein family

LC-MS/MS

2D-LC-MS/MS

Spot #

60S acidic ribosomal protein

gi|729348440

gi|802562610

27

Aldehyde dehydrogenase

gi|8163730

gi|238846406

38

Aspartic peptidase (Peptidase A1 family)

gi|976909721

gi|976909721

10

Bet v 1 family

gi|976909362

gi|167472849

3

Beta-1.3-glucanase (Glycosyl hydrolase 17 family)

gi|976496731

gi|976928968

30

Calreticulin

gi|976913141

gi|976913141

9

Cupin family (Germin-like protein family)

gi|976899679

gi|976927409

18

Cu-Zn superoxide dismutase

gi|976902802

gi|3914999

48

Cyclophilin

gi|488726160

gi|332806715

2

Cysteine protease

gi|1706276

gi|28804503

11

Cytochrome c family

gi|118012

gi|976899814

17

Defensin-like protein

gi|817033923

gi|817033923

1

Enolase

gi|3023685

gi|976924044

30

Glyceraldehyde-3-phosphate dehydrogenase

gi|752855788

gi|902550458

33

Heat shock protein 70

gi|698946330

gi|976917633

9

LDH/MDH superfamily

gi|976916739

gi|976905497

32

NAC-alpha family

gi|729420144

gi|976923178

6

Pathogenesis-related protein PR-1

gi|743838314

gi|976917157

51

Pectate lyase

gi|113476

gi|302127818

39

Thaumatin-like protein

gi|20385169

gi|20385169

1

Thioredoxin

gi|720041636

gi|976899454

36

Triosephosphate-isomerase

gi|976918965

gi|976923382

1

Tubulin family

gi|803378277

gi|902550499

49

Chitinase

gi|425886502

n.i.

—

Chlorophyll a-b binding protein

gi|146403796

n.i.

—

Cobalamin-independent methionine synthase

gi|976902600

n.i.

—

Flavodoxin

gi|720077294

n.i.

—

Glutathione S-transferase

gi|698502214

n.i.

—

GMC oxidoreductase

gi|659069842

n.i.

—

Heat shock protein 90

gi|702435110

n.i.

—

Iron/manganese superoxide dismutase

gi|15551753

n.i.

—

Isoflavone reductase

gi|923534270

n.i.

—

L3 Ribosomal protein

gi|901818290

n.i.

—

Lipid transfer protein

gi|118490068

n.i.

—

Pectin methylesterase

gi|976571059

n.i.

—

Peroxiredoxin

gi|823226051

n.i.

—

Polcalcin

gi|976896497

n.i.

—

Polygalacturonase (Glycosyl hydrolase 28 family)

gi|976926788

n.i.

—

Profilin

gi|62249502

n.i.

—

Protein kinase

gi|976914952

n.i.

—

Pyrophosphatase

gi|901817400

n.i.

—

Serine protease (Peptidase S8 family)

gi|976923421

n.i.

—

Alcohol dehydrogenase

n.i.

gi|970021885

39

Beta-xylosidase

n.i.

gi|85813770

30

Endochitinase (Hevein like-domain)

n.i.

gi|848864792

9

Serine carboxypeptidase (Peptidase S10 family)

n.i.

gi|976807531

11

Xyloglucan endotransglucosylase (Glycosyl hydrolases family 16)

n.i.

gi|82394883

9

  1. n.i., not identified.