Table 1 Specifications of antibodies used in this study.
Antibody (clone) | Region specificity | Antigen | Source | Use and Dilution |
|---|---|---|---|---|
Rabbit polyclonal to APP CT695 (CT695) | Human, mouse and rat FL-APP and CTFs | C-terminus 22 amino acid residues of β-APP peptide | Thermoscientifi, Waltham, MA, USA | Western blotting (WB) 1:1000; |
Mouse monoclonal to human Aβ1-16 (6E10) | human Aβ (hAβ) | Amino acids residues 1–17 of hAβ peptide | Biolegend, Dedham, MA, USA | ELISA capture: 4 μg/ml |
Biotinylated mouse monoclonal to human Aβ17-24 (4G8) | hAβ | Amino acids residues 17–24 of hAβ peptide | Biolegend, Dedham, MA, USA | IHC 1:500; |
Biotinylated mouse monoclonal to human Aβ1-40 (5C3) | C-terminus of hAβ1-40; does not crossreact with hAβ1-42 | C-terminal hAβ1-40 peptide | Nano tools, Teningen, Germany | ELISA detection: 0.5 μg/ml |
Biotinylated mouse monoclonal to human Aβ1-42 (8G7) | C-terminus of hAβ1-42; does not crossreact with hAβ1-40 | C-terminal hAβ1-42 peptide | Nano tools, Teningen, Germany | ELISA detection: 0.5 μg/ml |
Rabbit polyclonal to phosphorylated APP (Thr668) | Human Phosphorylated APP at Thr668 | Phosphopepitopes matching to residues neighboring Thr668 of human APP695 | Cell signaling, Danvers, MA, USA | WB 1:1000 |
Rabbit polyclonal to human BACE1 (ab2077) | Human and mouse BACE1 | Amino acids residues 485–501 of Human BACE | Abcam, Cambridge, MA, USA | WB 1:1000 |
PHF-1 monoclonal to phospho Tau | Human, mouse and rat phospho Tau | Epitopes matching to residues neighboring Ser396 and Ser404 phosphorylated sites | Prof. Peter Davies Albert Einstein College of Medicine, Manhasset, NY, USA | WB 1:1000 |
HT7 monoclonal to total Tau | Human specific | Human Tau between residue 159 and 163 | Thermoscientific | WB, 1:1000 |
Mouse monoclonal to β-actin (C4) | β-actin | Bird gizzard actin | Santa Cruz, Dallas, TX, USA | WB: 1:1000 |
