Table 1 Quantitative PCR primers sequence.

From: Therapeutic effects of long-circulating miR-135a-containing cationic immunoliposomes against gallbladder carcinoma

Gene

Forward (5′->3′)

Reverse (5′->3′)

miR-135a RT Primer

5′-CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTCACATAG-3′

miR-135a

CCAGGCTTCCAGTACCATTAGG

GTTTCCGAGAGAGGCAGGTG

HOXA10

CAACTGGCTCACGGCAAAGA

GCTGCGGCTAATCTCTAGGC

BCL-2

GTGTGTGGAGAGCGTCAACC

GCATCCCAGCCTCCGTTATC

ROCK-1

GGAGAAGGAGGAGGAGATCAGTAA

TAGCAACACTTGTAGAATCCGAGAG

GAPDH

GAGTCCACTGGCGTCTTCAC

TGCTGATGATCTTGAGGCTGTT

  1. Quantitative PCR primer sequences. U6 was used as an invariant housekeeping internal control gene for miR-135a, and GAPDH was used as an invariant housekeeping internal control gene for HOXA10, BCL-2, and ROCK-1.
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