Figure 2 | Scientific Reports

Figure 2

From: Lectin-mediated reversible immobilization of human cells into a glycosylated macroporous protein hydrogel as a cell culture matrix

Figure 2

Lectin-mediated cell adhesion. (A) 2*105 cells were seeded onto a hydrogel surface in the absence or presence of different YFP-LecB concentrations from 0 to 400 µM. Cells could adhere to the surface in the presence of cell culture medium for 24 h, followed by staining of cell cytoskeleton (red phalloidin-rhodamine) and nucleus (blue DAPI). Cellular surfaces were analysed with confocal laser scanning microscopy. Adhesion was detected for YFP-LecB concentration of 300 µM and higher. (B) Typical A549 cells in the presence of 50 and 400 µM of YFP-LecB. All bars represent the standard deviation. The significance was tested with a one way anova with alpha = 0.05.

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