Figure 4 | Scientific Reports

Figure 4

From: Lectin-mediated reversible immobilization of human cells into a glycosylated macroporous protein hydrogel as a cell culture matrix

Figure 4

Lectin release from the hydrogel. To test the reversibility of YFP-LecB from the macroporous hydrogel matrix, gels were decorated with YFP-LecB. Afterwards, decorated hydrogels were incubated with different concentration (0–1000 µM) of lectin binding sugars (D-mannose, L-fucose and D-galactose). After 2 h, the YFP-LecB concentration was measured via fluorescence in the supernatant. (B) To investigate the time which is needed to elute lectin from the hydrogel, hydrogels decorated with YFP-LecB were incubated with 400 µM of L-fucose and at regular intervals samples were taken from the supernatant and analysed for YFP-LecB fluorescence. All bars represent the standard deviation.

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