Figure 1
From: High-Resolution Imaging of a Single Gliding Protofilament of Tubulins by HS-AFM
HS-AFM observation of gliding MTs. (a) Schematic illustration of in vitro gliding assay of MTs on kinesins fixed to a mica supported lipid bilayer through streptavidin/biotin interaction. (b) Time lapse images showing gliding motion of a MT on the kinesin coated lipid surface. Scale bar: 500 nm, frame rate: 0.2 s/frame. (c) Observation of a gliding PF by using HS-AFM. Scale bar: 500 nm, frame rate: 0.2 s/frame. In (b) and (c), the pink/red arrows indicate change of position of gliding MT or short PF with time and white arrows show their moving direction. (d) Height profile of gliding PFs obtained from HS-AFM image as shown in the inset. Red points in the insets show two PFs.
