Table 1 PCR primer sequences and expected PCR product size.

From: Paracrine cyclooxygenase-2 activity by macrophages drives colorectal adenoma progression in the Apc Min/+ mouse model of intestinal tumorigenesis

Primer pair

Sense (5′-3′)

Antisense (5′-3′)

size (bp)

mCox-2 gDNAa

dACGCGTCGACGAATCTCAGCACTGCATCCTGCC

dCGACGCGTCTTCTGTTATGGAAGATGTTACATG

5728

mCox-2 cDNA

dTCAAAAGAAGTGCTGGAAAAGGTT

dTCTACCTGAGTATCTTTGACTGTG

296

cLysozyme cDNA

dGATCGTCAGCGATGGAAACGGC

dCTCACAGCCGGCAGCCTCTGAT

101

cβ-actin

dAGATGACACAGATCATGTTT

dTCCACATCACACTTCATGAT

511

cLys-Cox-2 gDNA

dCCACCTGCCACTGAATGGCT

dCGGAAGAGCATCGCAGAGGTG

602

cLys-Cox-2 cDNA

dTCAAAAGGCGTTCAACTGAGC

dCTCACAGCCGGCAGCCTCTGAT

351

real-time mCox-1 gDNA

dTGGAGATGACGGGTCTGTCTTAG

dACTTGTCTTCATCAGGAACAAAACTC

—

real-time mCox-2 gDNA

dGAATTTTTTTTCATGTAACATCTTCCATAA

dGGACAAACACCGGAGGAATCT

—

  1. aSense and antisense primers contain Sal I and Mlu I recognition sites respectively (italicized).
  2. m, mouse.
  3. c, chicken.
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