Table 1 Bacterial strains used in this study.
From: Role of SCCmec type in resistance to the synergistic activity of oxacillin and cefoxitin in MRSA
Strain | Relevant Features | Reference |
|---|---|---|
Plasmids | ||
pSR | Multi-copy plasmid encoding ccrA2 and ccrB2 genes of N315; TetR | |
pMAD | E. coli/S. aureus allelic exchange vector; AmpR, EryR | |
pMAD-MW2Δspa | 600b of up- and downstream regions of the spa gene of MW2 cloned into pMAD; AmpR, EryR | This study |
pMAD-SACOL0057 | SACOL0057 inserted between 650 bp of the up- and downstream regions of attB site of MW2 cloned into pMAD; AmpR, EryR | This study |
Escherichia coli | ||
DC10B | Δdcm in DH10B background; Dam methylation only; for cloning | |
Staphylococcus aureus | ||
RN4220 | restriction-negative derivative of 8325-4 | |
COL | HA-MRSA; TetS; SCCmec type I, ST250, pbla− | |
COL-S | COL with SCCmec type I excised, pbla- | |
MW2 | CA-MRSA; SCCmec type IV, ST1, pbla+ | |
COL type IV | COL with SCCmec type IV, pbla- | This study |
RN4220 pPBP4-J | pPBP4-J integrated into RN4220, insertionally inactivating pbpD encoding PBP4; EryR | |
COL pbpDmut | pPBP4-J integrated into COL, insertionally inactivating pbpD encoding PBP4; EryR; pbla- | This study |
MW2 pbpDmut | pPBP4-J integrated into MW2, insertionally inactivating pbpD encoding PBP4; EryR; pbla+ | This study |
COL type IV pbpDmut | pPBP4-J integrated into COL type IV, insertionally inactivating pbpD encoding PBP4; EryR; pbla- | This study |
MW2-S | MW2 with SCCmec type IV excised; pbla+ | This study |
MW2-SΔspa | MW2 with SCCmec type IV excised and deletion of spa gene; pbla+ | This study |
MW2-SΔspa0057 | MW2 with SCCmec type IV excised, deletion of spa gene and insertion of SACOL0057 downstream of orfX; pbla+ | This study |
