Figure 1
From: Computational design of a symmetrical β-trefoil lectin with cancer cell binding activity
(a) Molecular weight determination by analytical ultracentrifugation. Sedimentation velocity data were processed to reveal relative abundance c(M) of species with molecular weight M ranging up to 100 kDa. The plot shows the curve for M values from 500 Da to 60 kDa. No species were present other than monomer, with a predicted M of 16553 Da. (b) The circular dichroism of Mitsuba-1 (green) and MytiLec-1 (orange) compared. Both models show similar features expected of a structure containing β-sheet, but these are more pronounced for Mitsuba-1. (c) A ribbon diagram of MytiLec-1 (PDB 3WMV), showing both subunits of the dimer, one coloured cyan and the other from blue (N terminus) to red (C terminus). N-acetylgalactosamine ligands are shown as sticks, with carbon atoms coloured yellow, oxygen red and nitrogen blue.
