Figure 2 | Scientific Reports

Figure 2

From: Multiplex polymerase chain reaction for pathogen detection in donor/recipient corneal transplant tissue and donor storage solution

Figure 2

Diagram of method for comprehensive PCR analysis of pathogen DNA in extracted recipient corneal tissue, donor corneal tissue, and donor cornea storage solution. This method comprised the three following steps: (1) qualitative multiplex PCR and quantitative real-time PCR, (2) broad-range real time PCR, and (3) broad-range real time PCR. Method 1: DNA from the following pathogens was found in the extracted tissue, the donor tissue and storage solution: HSV-1, HSV-2, VZV, EBV, CMV, HHV-6, HHV-7, HHV-8, BKV, JCV and HBV. Method 2: broad-range real time PCR in the extracted tissue, donor tissue, and storage solution revealed the presence of bacterial 16s rDNA. Method 3: broad-range real time PCR in the donor cornea storage solution revealed the presence of Candida and Aspergillus 18s rDNA. BKV = BK virus; HBV = hepatitis B virus; CMV = Cytomegalovirus; EBV = Epstein–Barr virus; HHV-6-8 = human herpes virus type 6-8; HSV-1, 2 = herpes simplex virus type-1, 2; JCV = JC virus; Parvo B19 = human parvovirus B19; PCR = polymerase chain reaction; VZV = varicella-zoster virus.

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