Figure 6 | Scientific Reports

Figure 6

From: RETRACTED ARTICLE: Fibulin-3 promotes osteosarcoma invasion and metastasis by inducing epithelial to mesenchymal transition and activating the Wnt/β-catenin signaling pathway

Figure 6

Effect of fibulin-3 knockdown and overexpression on osteosarcoma cell proliferation and colony formation abilities. (A) Downregulated fibulin-3 markedly inhibited cell proliferation of the highly invasive subclone HOS-1, whereas upregulated fibulin-3 significantly promoted cell proliferation of the low invasive subclone HOS-29. (B) The colony forming efficiencies of fibulin-3-silenced cells were decreased, and conversely, upregulation of fibulin-3 could increase the colony forming efficiency of the low invasive subclone. (C) The colony images of non-infected cells, control shRNA infected cells and fibulin-3 shRNA infected cells were examined by soft agar colony formation assay. (D) The colony images of non-infected cells, control shRNA infected cells and pLVX-fibulin-3 infected cells were examined by soft agar colony formation assay. (Magnification × 200). *P < 0.05.

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