Figure 9 | Scientific Reports

Figure 9

From: RETRACTED ARTICLE: Fibulin-3 promotes osteosarcoma invasion and metastasis by inducing epithelial to mesenchymal transition and activating the Wnt/β-catenin signaling pathway

Figure 9

Effects of fibulin-3 knockdown and overexpression on EMT genes correlated to tumor progression. After lentivirus transfections, EMT markers, including E-cadherin, N-cadherin, vimentin, Snail Slug and Twist were measured by (A) Western blot (cropped blot) and (B) real-time q-RT-PCR in the lentivirus transfection systems. (C) H3K4me3 and H3K27me3 expressions were assessed by Western blot. Histone H3 was used as a loading control. H3K4me3 is associated with transcriptional activation, and H3K27me3 was responsible for turning off transcription. Ectopic expression of fibulin-3 only increased H3K4me3 modification but had no effect on H3K27me3 modification. (D) Quantitative chromatin immunoprecipitation was performed to assess H3K4me3 occupancy in pLVX-EFEMP1 cells. IgG was used as the negative control. “% input” indicated the ratio of DNA fragment of each promoter region bound by H3K4me3 to the total amount of input DNA fragment without H3K4me3 antibody pull-down. *P < 0.05.

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