Figure 5
From: Cardiac spheroids as promising in vitro models to study the human heart microenvironment
Doxorubicin-mediated apoptosis is inhibited by L-NIO. (a) Confocal analysis of hCSs and iCSs cultured with and without 20 μM DOX and 100 μM L-NIO and stained with antibodies against active caspase 3 (shown in red). (b) Statistical analysis (n = 3) of black-versus-white ratios of confocal images of hCSs and iCSs cultured and stained as described in panel (a). Data are presented as mean ± SEM. Scale bars: 200 μm. One-way ANOVA followed by Bonferroni’s multiple comparisons test. F (df 5, 12) = 1.153.
