Figure 6
From: Cardiac spheroids as promising in vitro models to study the human heart microenvironment
DOX toxicity is mediated via NOS in different cell types. (a) Comparison of DOX-mediated toxic effects in co-cultures from different cell populations (n = 6): (i) iCS generated from iCM, EC and iCF; (ii) iCMs combined with ECs only; (iii) iCMs combined with iCFs only; and iv) ECs combined with iCFs. 100 μM L-NIO was used to inhibit NOS activity. Two-way ANOVA followed by Tukey’s multiple comparisons test. F (df 7, 32) = 17.13. (b) Comparison of DOX-mediated toxicity in iCSs where eNOS was inhibited by using eNOS shRNA in specific cell types (n = 6). Scramble shRNA was used as control. *Relative to iCS; ♯within the same cell population. Data are presented as mean ± SEM. Two-way ANOVA followed by Tukey’s multiple comparisons test. F (df 4, 40) = 5.846.
