Figure 4

CD300f-IL-5 receptor cross talk regulates alternatively activated macrophage formation in adipose tissue. The expression of hallmark alternatively activated macrophage markers such as Arg1, Chi3l, Relm-α and Ccl24 were determined in the adipose tissue of wild type (WT), Cd300f ^−/−, Il5 ^Tg and Il5 ^Tg /Cd300f ^−/− mice by quantitative PCR and normalized to the house keeping gene hypoxanthine-guanine phosphoribosyltransferase (Hprt) (A,B and D) and ELISA (C). Furthermore, the expression of Arg1, Chi3l, and Ccl24 was determined in the primary adipose tissue macrophages that were sorted from Il5 ^Tg and Il5 ^Tg /Cd300f ^−/− mice by quantitative PCR (E–G). In situ proliferation of adipose tissue eosinophils, macrophages and monocytes was assessed in Il5 ^Tg and Il5 ^Tg /Cd300f ^−/− mice using flow cytometric analysis of EdU⁺ cells (H). Thereafter, total WAT and BAT macrophage levels in Il5 ^Tg and Il5 ^Tg /Cd300f ^−/− mice were assessed (I). Data in (A–D) are from n = 5–6 mice; in (E–G) n = 3; in (H,I) from n = 6; *p < 0.05, **p < 0.01, ***p < 0.001 as analyzed by Student’s t-test (A–I).