Figure 5
From: CD300f:IL-5 cross-talk inhibits adipose tissue eosinophil homing and subsequent IL-4 production
Viable adipose tissue endothelial cells express a CD300f ligand. White adipose tissue (A–D) and lungs (D) from wild type (WT) and Il5 Tg mice were obtained, enzymatically digested and stained with viability and endothelial cells markers (A). Thereafter, the binding of CD300f-IgG1 Fc fusion protein to viable adipose tissue (B,C) and lung (D) endothelial cells was determined by flow cytometry. Data are representative of n = 4 independent experiments; *p < 0.05, ns- non-significant as analyzed by Student’s t-test (C).
