Figure 7

Il5 ^Tg /CD300f ^−/− mice are protected from diet-induced weight gain and glucose intolerance. Wild type (WT), Il5 ^Tg and Il5 ^Tg /CD300f ^−/− mice were fed with high fat diet (HFD) for up to 12 weeks and their weight was monitored at the indicated time points (A,C). After ten (B) and twelve (D) weeks of HFD, the mice underwent glucose tolerance test (B,D). Il5 ^Tg and Il5 ^Tg /CD300f ^−/− mice were fed with NCD or HFD for twelve weeks. Thereafter, the white adipose tissue (WAT) was obtained, photographed (E) and weighed (F). In addition, adipocyte size was assessed in H&E stained slides (G–H). Data in (A–D) are representative of n = 12; in (E and G) photographs are representative of 6/12 mice; In (F and H), n = 14; *p < 0.05, **p < 0.01, ***p < 0.001 as analyzed by two-way ANOVA followed by Tukey post-hoc test (A–D) and Student’s t-test (F–H).