Figure 1 | Scientific Reports

Figure 1

From: PTEN/FOXO3/AKT pathway regulates cell death and mediates morphogenetic differentiation of Colorectal Cancer Cells under Simulated Microgravity

Figure 1

Simulation of microgravity induces cell clumping. The colorectal cancer cells DLD1 (a), HCT116 (c) and SW620 (e), when cultured in Rotary cell culture system at 10 RPM for 48 hours to simulate microgravity clump together to form large 3D structures (b,d,f) DLD1, HCT116 and SW620 respectively. Scale bar represents 200 μm. XTT assay of the microgravity simulated cells (g) shows the cell viability was significantly reduced. The experiment was performed thrice with individual controls **P < 0.005, *P < 0.05. Data represented as mean + S.D.

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