Figure 1 | Scientific Reports

Figure 1

From: Dysregulated miRNAs and their pathogenic implications for the neurometabolic disease propionic acidemia

Figure 1The alternative text for this image may have been generated using AI.

Volcano plot showing the results of the miRNA profile analysis in liver samples of wt and PA mouse. Following qRT-PCR, Ct values were assigned using the SDS2.4 software (Applied Biosystems, Thermo-Fischer) and Ct values above 37,0 were considered as non-detected. MicroRNAs showing very low responses (Ct > 35) in most of the samples of both groups were also removed for further analysis. Finally, Ct values were analyzed using the StatMiner software (Integromics, Perkin-Elmer). The integrated GeNorm software was used to check the stability of putative endogenous genes and a final number of seven was used for normalization (see materials & methods section). Relative comparison of gene expression was made using the wt samples as the reference for the Pcca −/−(A138T) group and the geometric mean of selected endogenous genes for normalization. Individual RQ (relative quantity) values as well as statistical p values were calculated for the detected 286 miRNAs and represented in log10 scale in the form of a volcano plot.

Back to article page