Figure 6
From: Physiological oxygen tension reduces hepatocyte dedifferentiation in in vitro culture
Blocking ERK1/2 and GSK-3β pathways reduce EMT in primary culture of hepatocytes. (A) Morphology of primary hepatocytes cultured in 21% O2 in the presence of ERK1/2 inhibitor (PD0325901, 10 μM) and GSK-3β inhibitor (CHIR99021, 10 μM). (B) Immunofluorescence staining of F-actin (red) in primary hepatocytes cultured in 21% O2 in the presence of PD0325901 (10 μM) and CHIR99021 (10 μM). Nuclei were stained with Hoechst.
