Figure 6 | Scientific Reports

Figure 6

From: Legionella pneumophila infection activates bystander cells differentially by bacterial and host cell vesicles

Figure 6

THP-1 cells respond to the CD63-negative EV fraction. (a,c) Pro-inflammatory THP-1 response depends on OMVs. 100 k pellets from L. pneumophila-infected or control THP-1 cells were generated. CD63-positive exosomes were removed by immunoprecipitation. Entire 100 k pellet or unbound EVs (CD63− fraction) were used for stimulation of THP-1 cells for 24 and 48 h. Expression of CXCL8 (a), CXCL8 secretion (b) and expression of miR-146a (c) were analysed. Data are shown as mean + SEM of three independent experiments. ns: not significant.

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