Figure 2 | Scientific Reports

Figure 2

From: CDK5 Regulatory Subunit-Associated Protein 1-like 1 Negatively Regulates Adipocyte Differentiation through Activation of Wnt Signaling Pathway

Figure 2

CDKAL1 suppresses adipocyte differentiation. (a–d) Overexpression of CDKAL1 in 3T3-L1 adipocytes. (a) Expression levels of Cdkal1 mRNA levels. (b) Plate view of Oil Red O staining (day 7). (c) Expression levels of adipocyte specific genes (n = 3, *p < 0.05, **p < 0.01, ***p < 0.001 vs control group by Student’s t-test). (d) Western blotting of CDKAL1, PPARγ, adiponectin and β-actin. (e–g) Knockdown of CDKAL1 by using short hairpin RNA (shRNA) targeting CDKAL1 (shCDKAL1) in 3T3-L1 adipocytes. (e) Expression levels of Cdkal1 mRNA levels. (f) Plate view of Oil Red O staining. (g) Expression levels of adipocyte specific genes (n = 3, *p < 0.05, **p < 0.01 vs control group by Student’s t-test).

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