Figure 6
Conserved cysteine residues in the amino terminal region of CDKAL1 are essential for its anti-adipogenic action. (a) Diagram of constructs used in the experiment. The numbers indicate the amino acid residues from the N-terminus of CDKAL1. (b) Plate view of Oil Red O staining of differentiated 3T3-L1 cells expressing CDKAL1 with point mutations (Ctrl, control). (c) Gene expression levels in differentiated 3T3-L1 cells expressing CDKAL1 with the point mutations (n = 3, *p < 0.025, **p < 0.005, ***p < 0.0005 vs control group by Dunnett’s test). (d) Western-blotting analysis of β-catenin protein in differentiated 3T3-L1 adipocytes expressing CDKAL1 with the point mutations. (e) Measurement of tRNALys(UUU) modification index in 3T3-L1 cells expressing shCDKAL1 (*p < 0.05, **p < 0.01 vs control group by Student’s t-test) and in 3T3-L1 cells expressing wild type CDKAL1 (n = 3).
