Figure 1 | Scientific Reports

Figure 1

From: Mycobacterial DNA-binding protein 1 is critical for long term survival of Mycobacterium smegmatis and simultaneously coordinates cellular functions

Figure 1

MDP1-KO rapidly dies after entering stationary phase. (a) Growth curve of M. smegmatis WT, MDP1-KO, and COMP strains over 30 days represented as colony forming units (CFUs)/ml of culture. Bacteria were grown at 37 °C in 7H9-ADC medium with stirring at 60 rpm under normoxia. Data is shown as a representative of duplicate experiments. (b) Western blot indicating the expression levels of MDP1 in WT, MDP1-KO (KO), and COMP strains at OD600 values of 0.5, 0.8, 1.0, and 1.2. Ponceau S staining of transferred protein on the membrane were shown to confirm the loaded samples have the same amount of proteins and protein bands proved with anti Hsp65 antibody were shown as the internal controls.

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