Figure 2

Protein spots over 3-fold-different in MDP1-KO compared with WT. Representative silver-stained two-dimensional gel electrophoresis of the cellular proteins of MDP1-KO (OD600, 1.2) and the 29 significantly differentially expressed spots (≥3-fold change) between MDP1-KO and WT gel replicates applied to nLC-MS/MS analysis were marked by circles. The horizontal axis represents isoelectric points (pI) ranging between 3–10 (right) and 4–7 (left), and the vertical axis denotes second dimension electrophoresis by molecular weight.