Table 1 Functional identification of the significantly differentially expressed proteins identified by nLC-MS/MS between WT and MDP-1 KO M. smegmatis strains.
Spot number | Protein name | Up/Down regulated | Fold change | UniProt Acc No. | Gene symbol | Molecular Function |
|---|---|---|---|---|---|---|
1 | Alkyl hydroperoxide reductase C | Up | 4.5 | Q57529 | ahpC | Antioxidant activity |
3 | Protein RecA | Up | 4.4 | Q5QJ16 | recA | Damaged DNA binding |
5 | Putative oligopeptide permease | Up | 4.2 | Q938E4 | Â | ATPase activity |
6 | 50S ribosomal protein | Down | 4.2 | O06115 | rplV | rRNA binding |
7 | Peptide synthetase | Up | 4 | Q9RLP6 | mps | Catalytic activity |
9 | Chromosomal replication initiator protein | Up | 3.9 | P0C557 | dnaA | DNA replication origin binding |
10 | Putative uncharacterized protein | Up | 3.8 | B3GNI8 | Â | Unknown |
11 | NADH dehydrogenase | Up | 3.8 | O52473 | ndh | Oxido-reductase |
12 | Beta-lactamase-like protein | Up | 3.7 | Q9X2P5 | bllp | Unknown |
14 | FolD | Up | 3.4 | Q79N51 | folD | Methylene-tetrahydro-folate dehydrogenase activity |
15 | 60 kDa chaperonin | Up | 3.4 | P80673 | groL | ATP binding |
16 | DTDP glucose-4,6- dehydrogenase | Up | 3.4 | Q8GJ79 | rmlB | Catalytic activity |
18 | Carbon monoxide dehydrogenase | Up | 3.3 | Q7X0G4 | coxL | Oxido-reductase activity |
19 | Putative oxidoreductase | Up | 3.2 | Q9AQD0 | Â | D-arabinono-1,4-lactone oxidase activity |
21 | 30S ribosomal protein | Up | 3.1 | P41193 | rpsG | rRNA and tRNA binding |
22 | Putative transporter protein | Up | 3 | Q9RPH4 | Â | Transmembrane transport |
23 | Fatty acid synthetase I | Up | 3 | Q6XXM0 | fas1 | Enoyl-[acyl-carrier-protein] reductase |
24 | Integration host factor | Up | 4.5 | P96802 | mIHF | Nucleic acid binding |
25 | Catalase-peroxidase | Up | 7 | A0A0C4N7T7 | katG | Catalase activity |
26 | Acetyl-/propionyl- coenzymeA carboxylase | Up | 5.8 | A0QTE1 | accA3 | Biotin carboxylase activity |
27 | Alcohol dehydrogenase | Up | 5.5 | A0QU52 | MSMEG_2079 | Oxido-reductase activity |
29 | Universal stress protein | Up | 3.3 | A0QZA1 | MSMEG_3950 | Response to stress |
