Figure 2
From: Non-uniform self-assembly: On the anisotropic architecture of α-synuclein supra-fibrillar aggregates
PLM, CLSM and CRM images of SFAs. Side view PLM images of SFA rotated at (A) 45°, (B) 0° and (C) 315° relatively to the polarization axis of the analyzer. Front view PL micrographs of an SFA at (D) 45° and (E) 0°. (F) Confocal z-stack slice, taken along the length of the cylinder, from the middle section of an SFA formed within the presence of αS140C-Al647 (red) and stained with the amyloid specific dye Thioflavin T (green). Scale bars are 10 µm. The dashed line guides the eye for the relative rotation of the aggregate with respect to the crossed polarizers. In the top row (A–C) the line coincides with the central axis of the aggregates. The red frame in the 40 × 40 μm transmission image (G) designates the field of view for the CR mode of the microscope. (H) Background pixels which have intensity below the threshold of 880 AU (I) are colored in black. All the pixels exceeding the threshold are color coded according to the color scale (above 880 AU) in (H).
