Figure 4 | Scientific Reports

Figure 4

From: Alantolactone induces apoptosis, promotes STAT3 glutathionylation and enhances chemosensitivity of A549 lung adenocarcinoma cells to doxorubicin via oxidative stress

Figure 4

ALT induces ER stress and mitochondrial dysfunction through oxidative stress. (A) A549 cells were treated with indicated concentrations of ALT for 12 h. Total cell lysates were extracted and subjected to Western blots for the expression of TrxR1, p-eIF2α, eIF2α, ATF4 and CHOP. Columns not sharing same superscript letters within the group differ significantly (P < 0.05). (B) A549 cells were treated with ALT as indicated for 12 h. Total cell lysates were extracted and subjected to Western blots for the expression of Bcl-2, Bax, and Bad. Columns not sharing same superscript letters within the group differ significantly (P < 0.05). (C) A549 cells were treated with 45 μM ALT in the presence or absence of 3 mM NAC for 12 h. Total cell lysates were then subjected to immunoblotting for the expression of Bcl-2, Bax, ATF4 and CHOP. Columns not sharing same superscript letters within the group differ significantly (P < 0.05).

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