Figure 7

Stereoscopic image of the EGFP-positive conceptus in the EGFP-negative uterus of wild-type mice. The EGFP-positive conceptus in the EGFP-negative uterus of wild-type mice was created by mating CAG-EGFP male mice with wild-type ICR female mice. Pregnant mice were transcardially perfused with 4% PFA and PI solution at E10.5. After the isolated reproductive organs were subjected to CUBIC, 3D images were taken using a light-sheet microscope, and stereoscopic images were reconstructed electronically. Scale bars, 1 mm.