Figure 2
Metabolite levels in UCHL1-overexpressing murine breast cancer EMT6 cells. (a–f) Metabolites extracted from EMT6 cells transfected with the indicated expression vector for none (EV: a–f), UCHL1 (a–c), or UCHL1 C90S mutant (d–f), and with the indicated shRNA for HIF-1α (shHIF-1α: a–c) or scramble negative control (shNC: a–c) were subjected to quantitative analyses of [13C3]lactate (a,d), [13C2]citrate (b,e), and [13C2]isocitrate levels (c,f). Means ± s.d. n = 3. *P < 0.05, **P < 0.01. NS, not significant (Student’s t-test).
