Figure 1
From: CD146 is required for VEGF-C-induced lymphatic sprouting during lymphangiogenesis
CD146 is expressed in LEC and regulates LEC activation induced by VEGF-C. (a) WB analysis of CD146 and VEGFR-3 expression in four different endothelial cell lines. (b) Serum-starved HDLEC cells were treated with VEGF-C156S (100 ng/ml) for different time intervals. The expression of CD146 and VEGFR-3 was determined by WB. (c–f) HDLEC cells, transfected with siRNA of control, CD146, VEGFR-3 or combination of CD146 and VEGFR-3, were subjected to spheroid sprouting assay (c), proliferation assay (d), tube formation assay (e) and transwell migration assay (f). VEGF-C156S was applied at the indicated concentration. Data were expressed as means ± SEM from 3 independent experiments (n = 3 in each group of c–f). Significant difference was determined by Student’s t-test (*P < 0.05; **P < 0.01; ***P < 0.001; ns, no significant difference). Full-length blots are presented in Supplementary Fig. S4.
