Figure 5
From: Strategies to Mitigate Variability in Engineering Human Nasal Cartilage
Effect of hypoxia and co-culture on tissue quality. (a). Top: Histological and immunofluorescence images for the worst donor (left, Bern Score = 1) in both normoxia and hypoxia. Bottom: Tissue generated with chondrocytes from the same donor co-cultured with BM-MSC in normoxia and hypoxia (b). Top: Histological and immunofluorescence images for the best donor (left, Bern Score = 8) Bottom: Tissue generated with chondrocytes from the same donor co-cultured with BM-MSCs. Fast green, Safranin O and haematoxylin staining showing the distribution of the collagen, GAG and cells respectively. Immunofluorescence images, red fluorescence is collagen I, green is collagen II and blue is DAPI for cell nuclei. Scale bar is 200 µm.
