Table 1 RT-qPCR Primer Sequences.
From: Strategies to Mitigate Variability in Engineering Human Nasal Cartilage
Gene | Accession # | Forward | Reverse |
|---|---|---|---|
ACAN | M_55172 | AGGGCGAGTGGAATGATGTT | GGTGGCTGTGCCCTTTTTAC |
COL1A2 | NM_000089 | TTG CCC AAA GTT GTC CTC TTC T | AGC TTC TGT GGA ACC ATG GAA |
COL2A1 | NM_033150 | CTG CAA AAT AAA ATC TCG GTG TTC T | GGG CAT TTG ACT CAC ACC AGT |
COL10A1 | X60382 | GAAGTTATAATTTACACTGAGGGTTTCAAA | GAGGCACAGCTTAAAAGTTTTAAACA |
SOX9 | Z46629 | CTTTGGTTTGTGTTCGTGTTTTG | AGAGAAAGAAAAAGGGAAAGGTAAGTTT |
YWHAZ | NM_003406 | TCTGTCTTGTCACCAACCATTCTT | TCATGCGGCCTTTTTCCA |
HOXC4 | NM_014620.5 | ACCGTCGCATGAAATGGAA | TGCTGACCTGACTTTGGTGTTG |
HOXC5 | NM_018953.3 | GGTGCAGGCATCCAGGTACT | CGGTGGGAAAGTGATGCTTAA |
HOXC8 | NM_022658.3 | AACCCGTGCTCGCTTAGCT | GCCTCGTAGCCATAGAATTTGG |
HOXD3 | NM_006898.4 | GGAGCTTCCTGAGTGCACAAT | CCTCCAAACAGTCCTGGGTTT |
HOXD8 | NM_019558.3 | GGAATTTCTTTTTAACCCCTATCTGA | GCTAGGGCGTGGGAAACC |
FGFR1 | NM_023110.2 | AACCTGACCACAGAATTGGAGGCT | ATGCTGCCGTACTCATTCTCCACA |
FGFR2 | NM_000141.4 | TGATGGACTTCCTTATGTCCGCGT | AGCGTCCTCTTCTGTGACATTGGT |
FGFR3 | NM_000142.4 | ACCAATGTGTCTTTCGAGGATGCG | AGAGCACGCAGCTTGTCACATAGA |
FGFR4 | NM_002011.3 | ATGGAACTGGTGTGCTCAAGAAGC | TTCACATGTCCTCCGACCAACACA |
