Figure 7

Determination of optimal dilution of HRP-labeled anti-Lv IgG (A), and a representative standard curve obtained for guppy Lv (B) and WBH dilution curves of control male and E2-exposed male guppy in sandwich ELISA (C).

Determination of optimal dilution of HRP-labeled anti-Lv IgG (A), and a representative standard curve obtained for guppy Lv (B) and WBH dilution curves of control male and E2-exposed male guppy in sandwich ELISA (C).