Table 1 Expression level of upstream EMT signalling in the tumour xenograft mice model by IHC combined semiquantitative scoring systems.
Maker | Placebo | CXB | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
66T | 44T | 54T | 57T | 69T | 65T | 50T | 49T | 61T | 55T | 48T | 71T | 58T | 37T | p value† | ||
Membrane EGFR | ||||||||||||||||
Intensity | 2+ | − | + | − | 2+ | + | 2+ | − | − | + | − | − | − | + | ||
Percentage | 85 | 0 | 80 | 0 | 40 | 10 | 70 | 0 | 0 | 10 | 0 | 0 | 0 | 30 | ||
Allred Score | 7 | 0 | 6 | 0 | 6 | 3 | 7 | 0 | 0 | 3 | 0 | 0 | 0 | 4 | 0.0322 | |
IRS Score | 8 | 0 | 3 | 0 | 4 | 1 | 6 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0.0326 | |
Nuclear FAK | ||||||||||||||||
Intensity | + | + | + | − | + | + | + | − | − | − | + | − | + | + | ||
Percentage | 80 | 60 | 60 | 0 | 90 | 60 | 50 | 0 | 0 | 0 | 30 | 0 | 50 | 30 | ||
Allred Score | 6 | 5 | 5 | 0 | 6 | 5 | 5 | 0 | 0 | 0 | 4 | 0 | 5 | 4 | 0.0113 | |
IRS Score | 4 | 3 | 3 | 0 | 4 | 3 | 2 | 0 | 0 | 0 | 2 | 0 | 2 | 2 | 0.0100 | |
Nuclear Slug | ||||||||||||||||
Intensity | 2+ | + | + | − | + | − | + | − | − | − | − | − | − | 2+ | ||
Percentage | 90 | 20 | 70 | 0 | 50 | 0 | 40 | 0 | 0 | 0 | 0 | 0 | 0 | 70 | ||
Allred Score | 7 | 4 | 6 | 0 | 5 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 0.0514 | |
IRS Score | 8 | 2 | 3 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 0.0439 | |
Nuclear Twist | ||||||||||||||||
Intensity | + | − | − | − | − | + | + | − | − | − | − | − | + | − | ||
Percentage | 70 | 0 | 0 | 0 | 0 | 80 | 80 | 0 | 0 | 0 | 0 | 0 | 30 | 0 | ||
Allred Score | 6 | 0 | 0 | 0 | 0 | 6 | 6 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0.0986 | |
IRS Score | 3 | 0 | 0 | 0 | 0 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0.0986 | |
Nuclear ZEB1 | ||||||||||||||||
Intensity | + | + | − | − | − | + | + | − | − | − | − | − | + | + | ||
Percentage | 60 | 10 | 0 | 0 | 0 | 60 | 60 | 0 | 0 | 0 | 0 | 0 | 30 | 30 | ||
Allred Score | 5 | 3 | 0 | 0 | 0 | 5 | 5 | 0 | 0 | 0 | 0 | 0 | 4 | 4 | 0.0497 | |
IRS Score | 3 | 2 | 0 | 0 | 0 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0.0422 | |
