Figure 6

Echinomycin inhibits adipogenesis in high-fat diet mice. (A) qRT-PCR in eWAT for a HIF-1 target gene, vascular endothelial growth factor (Vegf) in either normal-fat diet (NFD) fed mice or high-fat diet (HFD) fed mice (n = 6–8 in each group). Echinomycin was administered intraperitoneally at two doses and frequency (EC-Low or EC-High). See the Method for further information. **P < 0.01, as determined by a two-way ANOVA with Bonferroni’s post-test. (B) Body weight (BW) change in HFD fed mice groups. Echinomycin inhibited the BW gain (n = 6–8). (C) eWAT (mg)/BW in each group (n = 6–8). (D) qRT-PCR of leptin in eWAT (n = 6–8). **P < 0.01, as determined by a two-way ANOVA with Bonferroni’s post-test. (E) H&E staining and average size of adipocyte from eWAT (n = 6–8). Scale bar 30 um. **P < 0.01, as determined by a two-way ANOVA with Bonferroni’s post-test.