Figure 1

Electrophoretic profiles of EWs (with exception of CEW, DEW, GEW, PEW and QEW). EWs (1–3 μg per lane) were separated on NuPage 4–12% Bis-Tris gels and silver stained. The single capital letters above the gels illustrate the systematic order of birds while triple letters indicate abbreviated common names of birds (Table S-1). In each gel, 5 μL of molecular mass marker and 0.5 μg of purified chicken (CH) protein standards (OVT, OVA, OVM and LYZ) were separated (not shown for simplification). For comparison, the gels were aligned based on the migration of two marker bands: 97 (orange line) and 19 kDa (red line).