Figure 4
From: Apoptosis inhibitor of macrophage ameliorates fungus-induced peritoneal injury model in mice
Expression of AIM in the zymosan-induced peritonitis model. Expression of AIM was increased in the wild mice. (a) Immunohistochemistry revealed AIM in the area of peritonitis in AIM +/+ mice, this being most abundant in the necrotic areas (arrow heads). There was no expression of AIM in AIM −/− mice. Scale bars, 200 μm. (b) Western blotting revealed an increase in circulating AIM in serum from day 7 in zymosan model AIM +/+ mice. (c) Cd5l (AIM) mRNA assessed by real-time PCR was upregulated from day 7 in zymosan model AIM +/+ mice. CTL: control normal AIM +/+ mice, KO: AIM −/− mice. n = 6 for each group. (d) Double staining of F4/80 (green) and AIM (red) revealed that AIM was released from macrophages in the surrounding cell area. Blue indicates DAPI staining. Scale bar, 50 μm.
