Figure 2
From: Involvement of Monocyte Subsets in the Immunopathology of Giant Cell Arteritis
Altered distribution of monocyte subsets in GCA and PMR. (a) Flow cytometry gating strategy based on CD14 and CD16 expression to distinguish classical (CD14brightCD16neg), intermediate (CD14brightCD16+) and non-classical (CD14dimCD16+) monocytes subsets (left panel). Flow cytometry dot plots showing representative samples (equal numbers of events) from a healthy control (HC), a newly diagnosed GCA patient (nGCA) and a newly diagnosed PMR patient (nPMR) (right panel). (b) Absolute numbers of classical, intermediate, and non-classical monocytes in HC (HC, n = 20), newly-diagnosed patients with GCA (nGCA; n = 21) and PMR (nPMR; n = 19) and in the follow-up samples of GCA (rGCA; n = 14) and PMR (rPMR; n = 15) patients in remission after 3 months of glucocorticoid treatment. Data are expressed as Tukey box and whisker plots. The bottom and top of the box represent the first and third quartiles, and the band inside the box represents the second quartile (the median). The whiskers represent the 1.5 IQR of the lower and the upper quartiles. Outliers are plotted as dots. The Kruskal-Wallis test was performed to compare data among study groups. The Mann-Whitney U test was used to compare each patient group with HC. Paired samples were compared with the Wilcoxon signed rank test. P-values of less than 0.05 (2-tailed) were considered statistically significant.
