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Figure 1

From: Tailoring seed oil composition in the real world: optimising omega-3 long chain polyunsaturated fatty acid accumulation in transgenic Camelina sativa

Figure 1

Production of EPA and DHA in the seeds of C. sativa. (a) The conventional Δ6 biosynthetic pathway for the production of omega-3 LC-PUFAs EPA and DHA, with the various desaturase and elongase enzyme activities shown in different colours [also used in (b)]. (b) Simplified maps of vectors EPA _B4_1 and DHA_33_13 used for transformation of C. sativa. Abbreviations: Cnl, conlinin 1 promoter for the gene encoding the flax 2S storage protein conlinin; USP, promoter region of the unknown seed protein of Vicia faba; SBP, sucrose binding protein 1800 promoter; NP, napin; Ot∆6, ∆6-desaturase from O. tauri; Tc∆5, a Δ5-desaturase from Thraustochytrium sp.; Piw3, ω3-desaturase from Phytophthora infestans; Ps∆12, a Δ12-desaturase from Phytophthora sojae; EhΔ4, ∆4-desaturase from E. huxleyi; PSE1, a Δ6-elongase from P. patens; OtElo5, Δ5-elongase from O. tauri; OCS, 35S, E9 and CatpA represent terminators.

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