Figure 2

Total fatty acid composition (mol%) of laboratory and field-grown wild-type and engineered C. sativa seeds. Distribution of FAMEs in wildtype C. sativa grown (2015) in the field (a) and laboratory (b); the EPA producing line EPA_B4_1 grown (2015) in the field (c) and laboratory (d); and a comparison of the DHA producing line, DHA5_33, grown in the field in 2014 (e) and 2015 (f). An average of 75 seeds analysed by GC-FID (flame ionisation detection). Endogenous fatty acids are shown in shades of green; intermediates of the introduced biosynthetic pathway are shown in shades of red, and the key target fatty acids (EPA and DHA) are shown in shades of yellow.