Figure 5

Analysis of triacylglycerols from mature C. sativa seeds. TAG molecular species were characterised from wild-type, EPA_B4_1 and DHA5_33 using a ESI-MS/MS neutral loss survey scan with each TAG species represented by the total number of fatty acid carbon atoms:desaturations. A Students T-test was used to independently compare C. sativa wildtype with EPA-B4_1 and DHA5_33. Those TAG molecular species with significantly (p 0.05) different accumulations are shown (a). Further analysis of the mass spectrometry data acquired for the engineered lines identified those significant TAG species containing either EPA (b) or DHA (c).