Figure 1
From: In vitro significance of SOCS-3 and SOCS-4 and potential mechanistic links to wound healing
Expression profile and generation of SOCS-3 over-expression and SOCS-4 knockdown models. (a) RT-PCR screening of HaCaT and HECV cell lines for SOCS-1 (108 bp), SOCS-2 (461 bp), SOCS-3 (521 bp) SOCS-4 (541 bp), SOCS-5 (117 bp), SOCS-6 (131 bp), SOCS-7 (590 bp) and GAPDH (475 bp), negative control (-ve) represents amplification of molecular biology grade water instead of cDNA. Verification of SOCS-4 transcript knockdown was assessed and confirmed using q-PCR in (b) HaCaT and (c) HECV cell lines. Protein knockdown in (d) HaCaT and (e) HECV cells were verified using western blot analysis. Verification of SOCS-3 over-expression at transcript level was confirmed using q-PCR in (f) HaCaT and (g) HECV cell lines. Protein knockdown was verified in (h) HaCaT and (i) HECV cells using western blot analysis. RT-PCR and western blot data represents cropped images, full length gels and blots are included in supplementary information. Representative data of a minimum of three independent repeats shown, error bars represent standard deviation, * represents p < 0.05, *** represents p < 0.001, **** represents p < 0.0001.
