Figure 3

Impact of SOCS-3 over-expression on cell proliferation and attachment. Five day proliferation rates of (a) HaCaT and (b) HECV cells following SOCS-3 over-expression, percentage respective pEF6 control of at least three independent repeats are shown and error bars represent the standard error of the mean. Cell attachment was assessed using an ECIS based method over 4 hours post seeding in (c) HaCaT and (d) HECV. Data, representative of at least three independent repeats shown, mean change in resistance, detected by the ECIS system, was used to indicate cell attachment to the electrode and error bars represent standard deviation. A Matrigel matrix adhesion assay was also implemented to examine Matrigel attachment of (e) HaCaT and (f) HECV cells following SOCS-3 over-expression, mean percentage respective pEF6 controls of at least three independent repeats are shown and error bars represent the standard error of the mean. * represents p < 0.05, ** represents p < 0.01, *** represents p < 0.001 and **** represents p < 0.0001.